목록구분 | 소장기록 |
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식별번호 | F-BC-0027 |
표제 | Influence of GSTM1 genotype on association between aromatic DNA adducts and urinary PAH...2002 |
내용 | Influence of GSTM1 genotype on association between aromatic DNA adducts and urinary PAH metabolites in incineration workers Jeongmi Leea, Daehee Kanga, Kyoung-Ho Leeb, Masayoshi Ichibac, Jiusong Zhangc, Katsumaro Tomokunic, Eung-Soo Hwangd, Chung-Gyu Parkd, Mina Hae, SungGyun Kimb, Sang-Beom Hanf, Jae-Wook Choig, Eunil Leeg, Jae-yeon Jangh, Paul T Stricklandi, Ari Hirvonenj, Soo-Hun Choa, b, , Abstract Waste incinerating workers are exposed to various pyrolysis products including polycyclic aromatic hydrocarbons (PAHs). We examined their PAH exposure by assessing urinary 1-hydroxypyrene glucuronide (1-OHPG), as a measure of internal dose, and aromatic DNA adducts in peripheral white blood cells (WBCs), as a measure of biological effect dose. The potential effect of genetic polymorphisms of three enzymes involved in PAH metabolisms (i.e., CYP1A1, GSTM1, and GSTT1) on these exposure markers was also investigated. Twenty-nine employees including workers incinerating industrial wastes and 21 non-exposed on-site controls were recruited from a company handling industrial wastes in South Korea. Sixteen ambient PAHs were determined by GC/MSD (NIOSH method) from personal breathing zone samples of nine subjects working near incinerators. Urinary 1-OHPG was assayed by synchronous fluorescence spectroscopy (SFS) after immunoaffinity purification using monoclonal antibody 8E11. Aromatic DNA adducts in peripheral WBC were measured by the nuclease P1-enhanced post-labelling assay. Genotypes were assessed by PCR-based methods. Information on smoking habits and use of personal protective equipment were collected by self-administered questionnaire. Urinary 1-OHPG levels were significantly higher in workers handling industrial wastes than in those with presumed lower exposure to PAHs (P=0.006, by Kruskal–Wallis test). A statistically significant dose-response increase in 1-OHPG levels was seen with the number of cigarettes consumed per day (r=0.686, P<0.001). Smoking and GSTM1genotype were significant predictors for log-transformed 1-OHPG by multiple regression analysis (overall model R2=0.565, P<0.001), whereas smoking was the only significant predictor for log-transformed aromatic DNA adducts (overall model R2=0.249, P=0.201). Aromatic DNA adducts were significantly correlated with log-transformed urinary 1-OHPG level (r=0.31, P=0.04). However, the partial correlation coefficient adjusting for age, sex, and cigarette consumption was not significant (r=0.15, P=0.17). The significant association exists only in individuals with the GSTM1 null genotype (Pearson’s correlation coefficient, r=0.52, P=0.01; partial correlation coefficient adjusting for age, sex, and cigarette consumption, r=0.36, P=0.04). Our results suggest that the significant increase in urinary 1-OHPG in the exposed workers is due to higher prevalence of smokers among them, and that the association between urinary PAH metabolites and aromatic DNA adducts in workers of industrial waste handling may be modulated by GSTM1 genotype. These results remain to be confirmed in future larger studies. Keywords Polycyclic aromatic hydrocarbon; Incinerator; 1-Hydroxypyrene glucuronide; Aromatic DNA adducts; Genetic polymorphism https://blog.naver.com/free5293/220425261518 ※ 저작권 관련으로 원문은 서비스 되지 않습니다. |
기록물유형 | 문서류 |
기록물형태 | 논문 |
원본소장처 | ELSEVIER |
생산자 | Jeongmi Lee; Daehee Kang; Kyoung-Ho Lee; Masayoshi Ichiba; Jiusong Zhang; Katsumaro Tomokuni; Eung-Soo Hwang; Chung-Gyu Park; Mina Ha; SungGyun Kim; Sang-Beom Han; Jae-Wook Choi; Eunil Lee; Jae-yeon Jang; Paul T Strickland; Ari Hi |
생산일자 | 2002-02-15 |
키워드 | |
원본형태 | 전자 |
크기분량 | 88.8KB; 9쪽 |
언어 | 영어 |
관리번호 | 49958 |